Restriction endonuclease HindIII cleavage site map of bacteriophage P22
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چکیده
منابع مشابه
Restriction endonuclease Hin dIII cleavage site map of bacteriophage P22.
The 14 Hind111 cleavage sites on P22 DNA have been mapped. Hind111 cleavage sites were located relative to EcoRI sites by determining the molecular weights and map order of fragments produced by HindIII, or Hind111 and EcoRI digestion. Molecular weights were estimated from the electrophoretic mobility of fragments. The Hind111 fragment order was established by Hind111 cleavage of segments of th...
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‘l’l~e SWYW EcoKI restriction endonuclease cleavage sites in bacteriophage P%d DNA have been mapped. The cleavage site map of circularly permut.ed P22 linear DNA is a circle. The positions of EcoRI sites in the early region of the P22 genomc were determined by comparing products of EcoRI digestion of maturtx liaear P22 chromosomes with the EcoRI cleavage fragments of DNA of three XimmP22 hybrid...
متن کاملThe restriction endonuclease cleavage map of rat liver mitochondrial DNA.
Mitochondrial DNA from rat liver contains six sites for cleavage by the restriction endonucleases Hind III and EcoRI. A large stretch of DNA, comprising about 40% of the mitochondrial genome is not cleaved by either of the enzymes; eight cleavage sites are located on a DNA stretch of 35% of the genome length suggestive of an unequal distribution of the A - T baspairs over the molecule. The numb...
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Mitochondrial DNA of the ascomycete fungus Aspergillus nidulans, a circular molecule of 31 500 base pairs, is cleaved by restriction endonucleases Eco R I, Hind II, Hind III and Bgl II into 3, 7, 9 and 5 fragments, respectively. The relative positions of the cleavage sites could be mapped by analysis of fragments obtained by double enzyme digestions of whole DNA and by complete and partial redi...
متن کاملThe cleavage site of the restriction endonuclease Ava II.
We have determined that the type II restriction enzyme Ava II, isolated from Anabaena variabilis, recognizes and cuts the sequence (formula: see article). The eight Ava II sites of pBR322 have been mapped, as well as a unique site for Ava I.
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ژورنال
عنوان ژورنال: Virology
سال: 1979
ISSN: 0042-6822
DOI: 10.1016/0042-6822(79)90491-4